Hi there,

I want to color protein surface by electrostatic potential data calculated using APBS 1.5. I tried two methods in Chimera and got quite different results. The resulted images can be found in attachment.

In the first attached figure (fig1_ep surface) the surface was colored by the “Electrostatic Surface Coloring” tool, in the second attached figure (fig2_value atom) the surface was colored by the “Values at Atom Positions” tool. Same color palette (red: -35, white: 0, blue: +35) and potential file was used in both cases.

Thanks for your help.

Yu Zhou