Dear Vignesh, You are using the wrong part of the tool. Where the dialog says "Start Structure" you chould change that to "Join Models" instead. Then you need to hide ribbons and show atoms so that you can see the N and C atoms you want to connect with a peptide bond, e.g. commands ~ribbon disp Then you need to select the two atoms for this bond, Ctrl-click and Shift-Ctrl-click on them in the 3D view. Then proceed as described for the peptide bond option of Join Models here: <https://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/editing/editing.h...> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Resource for Biocomputing, Visualization, and Informatics Department of Pharmaceutical Chemistry University of California, San Francisco
On Feb 2, 2024, at 12:03 AM, Vignesh Nandhagopal via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:
Dear Chimera Help Team, I want to create a peptide bond. I followed this manuscript which I have attached along with this mail. I followed the steps of the manual I have used and I have seen a slight change in build structure. Kindly help me with this I am getting an error in the unrecognized protein code. Kindly help me with the steps. <Screenshot (51).png><Screenshot (50).png><Screenshot 2023-07-25 103649.png><T--UNSW_Australia--Daniel_Winter_Chimera.pdf>