Hi Antonio,

The error "ImportError: No module named replyobj" means Chimera cannot find its message logging module called "replyobj".  That suggests that your Chimera installation is broken, and does not appear to be a problem specifically with BLAST.  I would suggest installing the current Chimera version 1.16.

If that does not fix it, use the Report a Bug... button that should appear when this error message is shown.  Or if that button does not appear use Chimera menu entry Help / Report a Bug....

Tom


On May 31, 2022, at 8:05 AM, Antonio Díaz Quintana via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:

Dear all,

Recently, I stopped being able to perform a BLAST search from the SEQUENCE tool in UCSF Chimera. Is there anything I could do to fix this?

Log message follows:

Web Service: BlastProtein2 is a Python wrapper that calls blastp to search nr or pdb for sequences similar to the given protein sequence
Opal service URL: http://webservices.rbvi.ucsf.edu/opal2/services/BlastProtein2Service
Opal job URL: http://webservices.rbvi.ucsf.edu/appBlastProtein2Service16540092552761301435050
Traceback (most recent call last):
  File "C:\Program Files\Chimera 1.15rc\share\chimera\tasks.py", line 179, in runStatusCB
    task.statusCBList[0]()
  File "C:\Program Files\Chimera 1.15rc\share\blastpdb\ParserBlastP.py", line 434, in _statusCB
    self.finishCB(self.params, output)
  File "C:\Program Files\Chimera 1.15rc\share\blastpdb\gui.py", line 424, in _finish
    self.fetchColumnValues()
  File "C:\Program Files\Chimera 1.15rc\share\blastpdb\gui.py", line 449, in fetchColumnValues
    info = fetch_pdb_info(pdb_ids)
  File "C:\Program Files\Chimera 1.15rc\share\blastpdb\pdbinfo.py", line 151, in fetch_pdb_info
    import replyobj
ImportError: No module named replyobj

Best,

Antonio

-- 
Dr. Antonio J. Díaz Quintana
Instituto de Investigaciones Químicas
Universidad de Sevilla - CSIC
Avda. Americo Vespucio 49
41092 Sevilla
Spain

Phone: +34 954 48 9631
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