Dear all, 
I need to merge two maps in MATLAB using
alignment data from chimera. Inputs:  two maps of the same box size, origin. I use matrixget -  to report translation and rotation
matrices for the aligned map . Translation operation works well when transferred into MATLAB (briefly, translation matrix gets converted in eulers and pased on to tom_rotate command in MATLAB, TOM package). However the x y z translation coordinates (the fourth column top to bottom in matrixget - output) are way off the real translation when using tom_shift command in MATLAB, TOM.
I found out that if I open the rotated map
and fit it again, it gives close to correct translation
matrix.
 
What's going wrong here? Does anyone have a
solution how to overlay 3D arrays in MATLAB using chimera-reported transformation matrices?
 
Many
thanks,
Oleksiy 
 

 
Dr Oleksiy Kovtun

Research Fellow

Briggs group

MRC Laboratory of Molecular Biology

Francis Crick Avenue

Cambridge

CB2 0QH

UK



Tel: + 44 1223 267551

Email: okovtun@mrc-lmb.cam.ac.uk



Dr Oleksiy Kovtun
Research Fellow
Briggs group
MRC Laboratory of Molecular Biology
Francis Crick Avenue
Cambridge
CB2 0QH
UK

Tel: + 44 1223 267551
Email: okovtun@mrc-lmb.cam.ac.uk