Hi Christos,
The Gasteiger implementation in Antechamber (which Chimera uses for partial-charge calculation) ignores the formal charge specification  -- which is a bug that I believed I've reported to the Antechamber developers at least once but I guess I'll report it again.
If you must use Gasteiger charges, at least some of the alternatives include the PETRA server run by the Gasteiger group itself:
http://www2.chemie.uni-erlangen.de/services/petra/index.html

and OpenBabel:

http://openbabel.sourceforge.net/wiki/Capabilities

--Eric

                        Eric Pettersen

                        UCSF Computer Graphics Lab

                        pett@cgl.ucsf.edu

                        http://www.cgl.ucsf.edu



On Aug 2, 2007, at 12:29 PM, cdeligka@purdue.edu wrote:

Dear all,

I tried to calculate the Gasteiger charges of peroxynitrite anion using Chimera
(I tried the version that was recently released as well as some previous ones).
This is my PDB file:

HEADER
HETATM    1  O     2    2        1.225   0.000   1.819
HETATM    2  N     2    2        0.138   0.000   1.281
HETATM    3  O     2    2        0.138   0.000  -0.086
HETATM    4  O     2    2        1.359   0.000  -0.746

The formal charge of this molecule is -1.
For some reason Chimera generates the Gasteiger charges for the molecule but
these charges correspond to a formal charge of zero and not -1.
If I try the other method AM1-BCC the charges that are calculated do correspond
to a formal charge of -1.

In my calculations I do need the Gasteiger charges and so I cannot use the ones
that are calculated by AM1-BCC.

If you have any suggestions please let me know.

Are you aware of any other software that might be able to do this calculation?

Thank you all,

Christos



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