hi, when using the Planes tool in Volume Viewer, are the planes numbered from 0 or 1? I loaded a contour using the IMOD import, where I had drawn the contour on slice 12 of a volume; however, it appears that the plane that transects the contour in Chimera is '11' when using the Planes tool. I tried to test the enumeration by exceeding bounds (150 slices are in the volume, so was expecting an error for an entry of '150' if it was enumerated from zero), but it appears that slice indices outside the range of the loaded volume just default to the limit in either direction (typing in '200' just sets it to the highest-enumerated slice). -- ------------------------------------------------------------- Jeff Triffo Auer Group, Donner Lab, Lawrence Berkeley National Laboratory Raphael Group, Bioengineering Department, Rice University Medical Scientist Training Program (MSTP), Baylor College of Medicine phone (Berkeley): 510-486-7940 fax (Berkeley): 510-486-6488