Thanks a lot now it works. Tom Goddard wrote:
Hi Hernando,
You have it almost exactly right except one detail. The origin of your map as shown in the volume dialog Coordinates panel should be -300 -300 0. This says to place the corner of the map (grid index 0,0,0) at xyz position (-300,-300,0) which in turn makes the center of the map (grid index 75,75,75) at (0,0,300) since the grid plane spacing is 4. Fit your one copy into the map with this map origin, save PDB relative to map, edit helical symmetries into PDB file (rotating about z axis through 0,0,0), reload that PDB, and use the sym command and it will work.
In the future Chimera will have a tool so you can specify helical symmetry (pitch and axis) and avoid the file editing step.
Tom
-- ----------------------------------- Hernando Sosa Dept. of Physiology and Biophysics Albert Einstein College of Medicine 1300 Morris Park Av. Bronx NY 10461 phone (718) 430-3456 FAX (718) 430-8819 email hsosa@aecom.yu.edu -----------------------------------