I would like to make the Glu and Asp protonated, and the Lys and Arg uncharged.
While I was trying to do it by addH function with chosing the protonation sates option -> Asp, or chose His. The resulting protonation states of Glu and Arg were the same, as Glu deprotonated and Arg protonated.
How could I reach the right protonatation states as I expected?
Thanks
Qchao