HI Smith, Your process makes no sense to me. It is not possible to do both of these things (1) make values below 0.18 = 0 and (2) make values above 0.17 = 1, because values in the range 0.17-0.18 cannot equal both 0 and 1 at the same time. Also, your second command didn't work as expected because you need the "setMaximum" keyword to set a new maximum. As shown in the help, the "set" keyword just sets a a new minimum. <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html#threshold> Did you instead mean (1) make values below 0.17 = 0 and (2) make values above 0.18 =1 ? If so, instead try: vop threshold #0 minimum 0.17 set 0 maximum 0.18 setMaximum 1 However, it is unclear why you even want to do that. If you just want to make a new map with values inside the current map isosurface as 1 and values outside the isosurface as 0 (or vice versa) just use the "mask" command instead. <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/mask.html> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Feb 11, 2021, at 3:11 AM, Smith Lee <smith_lee123@yahoo.com> wrote:
Dear All,
I have a map (A.mrc). When I open it in chimera (the map should be #0), the Volume Viewer shows the Range is -0.618 to 1.63, the default Level is 0.175.
With command "vop threshold #0 minimum 0.18 set 0" I got another map (let us call it B.mrc, should be #1), the Volume Viewer shows the Range is 0 to 1.63 (understandable, the maximum value is still 1.63 as in A.mrc, the minimum value is 0 as for all values not higher than 0.18 has been set to 0), the default Level is still 0.175.
Immediately I run the command "vop threshold #1 maximum 0.17 set 1". Before I running the command, I guess all the density value should be either "0" or "1", because all the value lower than 0.18 has been set to "0", and all value higher than 0.17 has been set to "1".
However after I run the command "vop threshold #1 maximum 0.17 set 1", I got another map (let us call it C.mrc, should be #2),the Volume Viewer shows the Range is 0 to 0.17 rather than 0 to 1, and wen I change the Level, the image of #2 changes correspondingly with the Level from 0 to 0.17.
Here I would ask, why the command "vop threshold #1 maximum 0.17 set 1" did not lead to a map with all values greater than 0,17 as 1? How to get a map based on A.mrc so that all values less than 0.18 as 0 and all values greater than 0.17 as 1 (please tell me the specific method)? Is any significance for the obtained C.mrc with range from 0 to 0.17?
I am looking forward to getting your reply.
Smith