Hi Chimera Users

This is 2^nd send to you after I enrolled as a CHIMERA user J, thanks.

I need to calculate a radial distance plot from a surface representation of an Adeno Associated Virus structure as shown from literature below.

However the:

1. Action, Surface, Show

command fails, reporting CHIMERA often fails for large structures ( AAV is easily over 200K atoms ) with multiple chain IDs ( I have 60 individual chain IDs for each of the 60 capsid proteins ).

Other CHIMERA popup suggestions did not resolve the problem either ( changing molecular surface parameters in the Inspect Selection ( actually didn’t see anything to change ) or in PREFERENCES - NEW SURFACES. I tried using CHIMERA, Structure editing, change chain IDs to all chain A, but that was disallowed.

Summary: could not figure out how to get CHIMERA to calculate and display a surface representation of an AAV virus and then use that display to calculate radial coloring as shown below.

Any solutions greatly appreciated and thanks.

Rob

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Robert Gampe, MS | Senior Scientist | RDRU Structural Biology| Pfizer Inc. 

7030 Kit Creek Rd, Morrisville, NC 27560

Office: 919-666-5969| Robert.Gampe@pfizer.com

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