It may be a function of the map display style. If I open a map from EMDB, e.g. commands:
open emdbID:1024
stereo anaglyph
I can clearly see the 3D effect when wearing red-cyan glasses (and red and cyan fringes with the glasses taken off) if I am showing surface or mesh isosurfaces for the map, but not if showing the diffuse cloud of the "solid" style. I attach an image of the map in surface style.
I don't know if you can expect to see a 3D effect with the transparent "solid" style even with anaglyph stereo working correctly. I couldn't perceive it with that style.
Tested Chimera 1.14 on Mac, and I believe that the map from EMDB is 32-bit floating point like your data.
I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco
<anaglyph.png>
On Sep 22, 2020, at 10:03 AM, Greg Couch <gregc@cgl.ucsf.edu> wrote:
Yes, the red and gray is normal, but you're seeing the combined channels. You need the glasses to separate the channels. Try viewing the image with the glasses that come with a 3D comic book.
-- Greg
On 9/21/2020 11:43 PM, Michael Elbaum wrote:
Red-cyan, sorry. No, I didn't look for stereo at all, just to reproduce the condition posted that one channel appears in red and the other in gray. Is that normal?
Michael
From: Greg Couch <gregc@cgl.ucsf.edu>
Sent: Tuesday, September 22, 2020 4:57:50 AM
To: Michael Elbaum <michael.elbaum@weizmann.ac.il>
Subject: Re: [Chimera-users] Stereo Red Cyan
Are you wearing red-cyan glasses to see the stereo? It doesn't work without them.
-- Greg
On 9/21/2020 1:08 PM, Michael Elbaum wrote:
I'm confirming the problem with a different dataset (optical deconvolution in tif format). Red-green shows red and gray, green-magenta shows green and gray. This is with version 1.14 on linux.
regards,
Michael
From: Chimera-users <chimera-users-bounces@cgl.ucsf.edu> on behalf of Angus McDonald <amcdonald@boisestate.edu>
Sent: Monday, September 21, 2020 9:46:24 PM
To: chimera-users@cgl.ucsf.edu BB
Subject: [Chimera-users] Stereo Red Cyan
I am looking at some cryo em data. When I try to view a a 3D stack of em dat with the Red-Cyan stereo setting I seem to only get Red and grayscale. The data is in 32-bit floating point and is in MRC2014 header format. I cannot figure out what I am doing wrong. Is anything obvious?
Thanks for your time.
--
Angus McDonald
amcdonald@boisestate.edu