Hi There,

I hope you are doing very well. My name is Roksana Azad, I am a Ph.D. Student at the CUNY ASRC.

I have been working on building a full length model of my protein into a cryoEM map, I have models for individual domain but not for the full lenght structure. I am trying to join the domain models using chimera "join models" function, but unfortunately it's not working for me. I tried everything I could find online about the issue, I used the addh command and kssdssp commands and also tried joining them using other bond instead of C-N, however my button for "apply" always grey and I can't click on it (please see the screenshot attached).

I also tried this command "#0:127.A@C #1:1.A@N" according to my model # and atom, but its not selecting anything for me. I tried "combine" it also didn't work.​

Please let me know if there is something else I am missing or what else I can do to create this bond or connection between two models.

Thank you so much for your time and help in advance – very much appreciated.

Sincerely,

Roksana