Dear Sujith, This page lists possible workarounds to surface calculation failures: <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/surfprobs.html> Sounds like you already tried #1 and #2 in that page, but the “molmap” method (#3) should always work, as it uses a completely different calculation. As to the other methods and whether they are needed, it depends on how you made the assembly. If I use the following commands, I get a tetramer from 1Q90 and each monomer is already a separate model, so there is no reason to split. I can show the surfaces without any problems on my machine, but perhaps it is a numerical failure on yours: open 1q90 sym #0 focus rainbow models surface If I open the model panel, I can see that the original model is #0 and the additional copies are models #1.1, 1.2, 1.3. I don’t know which two are the dimer you want, but if the above works for you, you can just hide or close the models you don’t want. I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco On Dec 3, 2015, at 6:14 PM, Puthiyaveetil, Sujith <s.puthiyaveetil@wsu.edu> wrote:
Hi Chimera developer, I encounter a problem with surface calculation/display when using the biological assembly (dimer) of 1Q90. The monomer on its own, as in pdb file 1Q90, displays the surface with no problem. I have tried the “split” command without success on the biological assembly. Also tried changing parameters to no effect. Would you please help. Regards, Sujith