Hi Alan, The Join Models section of Build Structure will work if you have the two parts (protein and thioester molecule) opened as two separate models, i.e. each was opened from a different file. Of course, you have to use the "other bond" option because it is not a peptide bond. <https://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/editing/editing.h...> If they are already in the same model as each other, then you can just add a bond between atoms using the Adjust Bonds section of Build Structure... however this will not move the two parts relative to each other, it will just add the bond in their current positions. <https://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/editing/editing.h...> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On May 6, 2023, at 12:07 PM, Alan Carbajo via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:
Hi Eric,
Thank you for the reply. A follow up question, I have a protein with a CYS whose sulfur forms a thioester and a carbon chain. I have a pdb for the large protein, and the separate thioester molecule that forms. Is it possible to replace the current S on the CYS and join it with the new one? I noticed the join molecule button doesn't work for it and the only thing I can do is build it using the add button.
Thank you!
Alan