Dear Neha, Now you tell me that you opened 1qwa as the pdb (atomic structure) file, so this mrc file is completely irrelevant. I believe you just used the wrong steps to save the pdb. You need to use menu: File... Save PDB, and in that dialog "Save models" area, choose the atomic structure model. (Hiding the map does not matter, centering the view does not matter, it is only what you choose in the dialog). Then as Tom already said, you also have to turn on the "Save relative to model" option and chose the map model as the one you want to save the PDB relative to. Here is the help for the Save PDB dialog: <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/savemodel.html#pdb> I hope this helps, Elaine ---- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On May 10, 2021, at 9:15 PM, Neha Goel <nehagoel24march@gmail.com> wrote: Dear Elaine, Thank You. Here I .mrc is attached. I was using a reference pdb like 1qwa from pdb database. Regards Neha Goel