Hi Francesco, You just need to get the correct chains paired up for morphing. Chain pairing is described here: <http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/morph/morph.html#pairing> In this case, with both structures having chains A-D, the automatic pairing for morphing is A with A, B with B, etc. However, that is not the same pairing that you have spatially. Spatially you have A on top of C (and vice versa), B on top of D (and vice versa). So before doing the morphing, you need to change the chain IDs of one of the structures so that A is on top of A, B is on top of B, etc. There is a Change Chain IDs tool in menu under Tools... Structure Editing. <http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/editing/changechains.html> However, in this e-mail message I can describe it more concisely with a command. I believe this will do the necessary renaming in your case: changechains A,B,C,D C,D,A,B #1 <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/changechains.html> Then you can make the morph correctly. Happy teaching, and you stay safe and well too! I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Mar 29, 2020, at 3:28 AM, Francesco Malatesta <francesco.malatesta@uniroma1.it> wrote:
Hello I am trying, for teaching purposes, to morph deoxy hemoglobin 1HBA and oxy hemoglobin 1BBB to show the O2-linked quaternary change. I get an obviously wrong morph, attached. Chimera is probably pairing different subunits? Do you think this morph can be done? Thank you Stay safe and well Francesco