Hi Filip, Another way to do it, if you are somewhat comfortable with python, is to use sparx, as described on this page: http://sparx-em.org/sparxwiki/align_chimera This will give you the Euler angles which describe the rotations, and can be converted to most of the common angle conventions (spider, EMAN, imagic, ...). Bill Filip Van Petegem wrote:
Dear chimera users,
I have a problem with saving a cryoEM map after rotating/translating it. I've used the 'fit in map' option to superpose 2 maps (in ccp4 format) This seems to work fine, but when I save the reoriented map (in any of the available formats), and try to open it, it is back to the original position.
Is there any way to save the reoriented map file?
Best regards,
Filip Van Petegem
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