Hi Andy, Probably the adjustments to the coordinates caused them not be recognized as "helix" - however, you can force them to be labeled as helix as follows: - select all the residues you want to be labeled as "helix" (there are many different ways, and it depends on the details of your situation which is easiest... if your copies all have the same residue numbering, you could do something like use the command "select : 43-57.a,103-119.b" which would select residues 43-57 in chain A and 103-119 in chain B in all models... but there are many other possible routes) - open the Selection Inspector (by clicking the greenish button on the bottom right corner of the Chimera window or choosing menu item Actions... Inspect) -- in that dialog, Inspect "Residue" and change "in helix" to "true" I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. meng@cgl.ucsf.edu UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco http://www.cgl.ucsf.edu/home/meng/index.html On Jan 14, 2009, at 12:45 PM, Anindito Sen wrote:
Dear All I have docked the crystal structure of a protein monomer manually in the density map ( of a filament) and with some movements done with a long alpha helix(part of the crystal structure) to make a decent fit. However when I generated multiple copies of the docked structure- regions where manual adjustments on the alpha helix were made- came out like thin wires instead of being a ribbon. Thanks Andy