Hi Elaine, Thank you for the quick response. I was doing my work via windows, not the command line. Is there something I need to do to keep all of the surfaces/sequences open??-Danny On Friday, May 13, 2016 3:57 PM, Elaine Meng <meng@cgl.ucsf.edu> wrote: Hi Dan,I wasn’t able to reproduce this problem. I just tested in Chimera 1.10.2 on 3 related proteins, with commands: open 2mnropen 1mucopen 4enldelete :.bmm #0 #1mm #0 #2tilesurf … then invoking Coulombic Surface Coloring, just clicking Apply because all three surfaces were already chosen in the dialog. Image of the result attached (after applying publication preset 1). I can’t think of any reason your system would behave differently, and all I can say is to make sure all the surfaces are chosen in the dialog. However, if that still doesn’t work you can always try submitting a bug report (menu: Help… Report a Bug) and attach the session so we could try to reproduce it. Best,Elaine----- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco On May 13, 2016, at 11:10 AM, zona cat <azrucu@yahoo.com> wrote: Good afternoon, My name is Daniel Heller, and I am working with Helen Berman at Rutgers University on a PDB project. We are trying to analyze multiple DNA binding proteins simultaneously (via matchmaker) and looking to visualize the surface electrostatic potential of each structure as a tiled picture. We are deleting the nucleotide chains for all proteins when complexed with DNA. However, I am only able to get the surface electrostatic coloring for one structure (the reference structure), subsequent structures will not apply the columbic coloring after i hit apply, they just maintain the monotone default surface structure . Could you please help me with this issue. Thank you very much, Dan
