Elaine Thank you for your repl;y. I found another structure that is complete 1GTR Thanks for your information. art -----Original Message----- From: Elaine Meng [mailto:meng@cgl.ucsf.edu] Sent: Wednesday, August 23, 2017 8:32 PM To: Arthur Szabo <agszabo@outlook.com> Cc: chimera-users@cgl.ucsf.edu BB <chimera-users@cgl.ucsf.edu> Subject: Re: [Chimera-users] secondary structure ribbons not drawn for CA-only protein Hi Arthur, PDB entry 1gsg contains only 1 atom per residue, CA atoms for the protein and P atoms for the tRNA. Chimera does not draw the secondary-structure ribbons for alpha-carbon-only proteins because it uses the carbonyl oxygens to define the plane of the ribbon. Some other program was used to make the image you see at the RCSB PDB. You could instead try using some other structure that is more complete. Here are all the structures with the same protein as 1gsg, most of which are complexed with tRNA (4jyz, 4jxx, etc.): <http://www.rcsb.org/pdb/results/results.do?tabtoshow=Current&qrid=841BDC61> I got this list by first searching RCSB PDB for 1GSG and going to its page: <http://www.rcsb.org/pdb/explore/explore.do?structureId=1gsg> … then lower in the page in the Macromolecules section, clicking the link to search for PDB structures with UniprotKB Access Code “P00962” I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Aug 23, 2017, at 4:03 PM, Arthur Szabo <agszabo@outlook.com> wrote:
I am interested in a structure 1GSG deposited in 1990. The PDB index for this file shows the enzyme Gln tRNA synthetase with the usual ribbon structure, bound to tRNA(Gln).
When I fetch 1GSG using either the PDB file number only, or that file number input into PDB biounit, the structure that comes up does not have the helical or β-sheet secondary structures shown as ribbons. Rather the entire protein backbone is shown without any secondary structure definition. One can see the helical segments in the worm like structure, but not in the form of the usual helical secondary structure.
How can I retrieve a structure that is similar to that in the PDB index list.
I have enclosed the following:
<image001.png>
This is what comes up when I fetch the PDB file.
<image002.png>
Thanks
Arthur G. Szabo Professor Emeritus