Re: [Chimera-users] fitting a protein inside an EM reconstruction

Hi Jayant, The reason that pressing the Fit button on the Fit Models in Maps dialog is not doing anything is because you have no atoms selected. A warning about this that says "No atoms selected" appears in the dialog. The fit only applies to models with selected atoms because that allows you to fit one PDB model at a time when you have multiple ones opened. The fit tool only does a local optimization -- relatively small rigid rotations and shifts -- so you have to first move the PDB model by hand to approximately where you want it. How to do this is described in the Guide to Volume Data Display: http://www.cgl.ucsf.edu/chimera/tutorials/volumetour/volumetour.html#fitmode... Tom