Hi Wil, Have you tried ConSurf server? http://consurf.tau.ac.il I haven't tried for a long time, but I think it can perform a sequence analysis using the sequence associated to the PDB ID (3V0I for you) and then it will map the residue conservation on to the structure. One the files in the results page will be a kind of Chimera session file. You need to download and open it in Chimera, then you’ll see the highlighted residues according to the conservation score. Regards, Aldo 2018-02-12 11:38 GMT+00:00 Wil Ratzan <wilratzan@gmail.com>:
Hello,
I truly appreciate the development and distribution of software such as Chimera to the scientific community, but alas I am not 'computer-savvy.'
I would like to map highly conserved residues of a protein onto its crystal structure (PDB 3V0I). I can view the PDB file of the crystal structure using Chimera, but I am having a hard time figuring out how to highlight residues. Could you please give me some advice about how to identify and mark particular residues using this file?
I apologize if this request seems trivial, and I appreciate any advice.
Thanks,
-Wil Ratzan
Dept. of Cell Biology and Neuroscience Montana State University Bozeman, MT 59717
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-- ========================================= Aldo Segura-Cabrera Chemogenomics Scientist European Bioinformatics Institute (EMBL-EBI) Wellcome Trust Genome Campus Hinxton, Cambridge, UK e-mail: asegura@ebi.ac.uk <asegurac@ipn.mx>; aldosegura@gmail.com website: http://aseguralab.org/ =========================================