Dear Huabin, You could save a Chimera session, but you'd also need to keep the input tomogram files and density map files in their current locations since the session file does not include them. <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/sessions.html> For saving multiple maps to one file, I believe the only choice is "Chimera map format" <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/volume.html#output> <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/volume.html#chimap> HOWEVER, as I understand it, simply saving a map to a file does not preserve any rotations and translations of that map such as from fitting. So first you may need to resample each density map to create a new density map in the new orientation, as discussed here: <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/savemodel.html#afterfitting> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Dec 13, 2021, at 11:59 AM, Huabin Zhou via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:
Dear Chimera team and users, I am trying fit hundreds of protein density maps into a tomogram by manually adjusting their positions. After the placement, I found I can't save all the maps together in a single mrc file. Is there anyway to do so? Or is there a way to extract the relative orientations of the individual models? Thank you! Best, Huabin