Respected Sir

I am junior research fellow in India. I am working with Chimera.

I have a problem.

I can see my EM (cryo EM) density map using Chimera. I want to fit the X-Ray cryptographic map with this EM density map. But when I open these two maps in Chimera window, they are not same size.  X-Ray cryptographic map is smaller than EM density map. How can I make it same size?

Size of  EM density map is 48,48,48

Level  is .0564 ( at this level my EM density map is looking good)

I also see your tutorial “Working with Volume Data: Chaperonin”. There you suggest if 23.5 A chaperonin density map and 2.8 A is protein crystal structure then adjust map level to around 0.09.

How could you calculate this “map level” value? 

Resolution of my EM density map is 22A⁰ and resolution of crystal structure is 2.3 A⁰ . What is the “map level value”?  I can not increase my map lavel value more than 0.650.

Thanking you.

With regards

Somnath Dutta

JRF

INDAI

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