Hi Weimin, I wonder if a 3d brush will be useful. The problem is you cannot see the depth in a 3d view of the data clearly. Amira provides a 2d brush which is much less trouble. The painting tool I suggested would have you push a button to paint within a sphere (maybe some other shape) and it would be a simple matter to allow you to drag it like a brush without the button press to test if that is useful. I'm not sure I understand the rotation and translation parameters you want. Is it that you have one map fit within another in 2 orientations and you want the rotation and translation relating those 2 orientations? Tom Weimin Wu wrote:
Thanks for your reply. I will try the eraser ball again. To add the density, I really need something like 3D brush. I have tried a series of Markers and split by colors, however it is hard to choose marker position and choose color zone radius, because we need separate the signals within 4A distance, especially when two subunits cross over and one beta sheet is very close to the beta sheet on the other subunit. For statistical comparison of two maps, I need not only correlation, but also rotation degrees, translation, especially for the hinge region, something like rmsd in pdb comparison.
Thanks, weimin