I used the automated H-bond detection of Chimera to detect H-bonds between an target protein and its docked lignds.
"[...] Relax H-bond constraints indicates that tolerances to
Relax constraints by should be applied to the precise
geometric criteria [...];
empirically, tolerances of
0.4 angstroms and
20.0 degrees
work well for most macromolecular structures."
Can you lease tell me if there is a literature which discusses this relaxation in more detail and can be referenced
Many thanks for a reply.