Hi Amit, You cannot model a multichain complex with the Chimera interface to Modeller, only a single chain.
From the manual page: <http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/multalignviewer/mo...>
"With this interface, *only a single chain or subunit can be modeled at a time.*" However, with the Modeller interface in our newer program, ChimeraX, you could model a multichain complex if you have the following required inputs: (a) a multichain complex structure to use as the template (the calculation will not guess how to arrange the proteins, it has to be provided in the template) (b) a target-template sequence alignment for each different protein in the complex, e.g., if a homomultimer you only need one sequence alignment See the ChimeraX User Guide for details and instructions. <http://rbvi.ucsf.edu/chimerax/docs/user/tools/modeller.html> ChimeraX is free for noncommercial use, like Chimera <http://www.rbvi.ucsf.edu/chimerax/index.html> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Aug 15, 2020, at 12:41 PM, amit gaur <cdriamitgaur@gmail.com> wrote:
Hi, I am trying to model a multichain protein complex using modeller extension in chimera. But, I do.nt know how to do that? Can some one help me with this ? Thanks -- Dr. Amit Gaur