I have an EM map for a virus that includes an antibody bound to the envelope proteins. The envelope proteins are trimers of dimers with
C3 symmetry that form spikes on the surface of the virus. I fit an atomic model for the spikes to the map using icosahedral symmetry, following the very helpful
symmetric fitting video tutorial. Now I am trying to fit a model for the antibody into the spike regions of the map. I need to impose C3 symmetry for the antibody for the fit
into each spike, in addition to imposing icosahedral symmetry to include all the spikes. How can I impose both of these in the fit?
Thanks,
Mike Kent