Thank you for your kind reply. Could you help me a little more? Where must the heme.defattr file be, when I use the 'defattr' command? I have tried to put it in the root directory, but it didn't work and I got such a message:

I have also tried to open this file using the tool 'Define Attribute' in Tools/Structure Analysis/, but got such a message:

пн, 14 сент. 2020 г. в 19:07, Elaine Meng <meng@cgl.ucsf.edu>:

The heme.defattr file is a link from the URL you already found:

> https://www.cgl.ucsf.edu/pipermail/chimera-users/2019-July/015972.html

In the lower part of that page, the link to the defattr file is

URL: <http://plato.cgl.ucsf.edu/pipermail/chimera-users/attachments/20190715/ecb1dce6/attachment.obj>

...and you just need to click that and then save it as a plain text file. You can rename that plain text file heme.defattr if you want.

I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco

> On Sep 12, 2020, at 9:53 AM, Rio Aquarius <rioxel@gmail.com> wrote:
>
> Hi,
> I have an issue with heme minimization, Fe atom leaves a heme plane. I saw at least one user had the same issue and got help to solve it (https://www.cgl.ucsf.edu/pipermail/chimera-users/2019-July/015972.html). I could try the protocol described there, but I don't have the heme.defattr file. I hope you can help me with it too. I have attached a pdb file with this issue.
> <CYP51_min300.pdb>