
13 Nov
2013
13 Nov
'13
12:20 p.m.
Dear Chimera Help, I have used NCBI's COBALT to align the active site residues for the ~400 kinases in the 'kinome tree'. This fasta alignment can be exported and read into chimera. Once loaded, tools > sequence > multialign/viewer > info > percent identity gives me the % identity for a pair of sequences. Since I have ~400 sequences, I would like to know whether the upper-triangular matrix of percent identities can be done automatically. If so, can a weight matrix for scoring (BLOSUM62) be used? Thanks in advance for your help. David Covell, Ph.D.