Tom, I don't suppose there is a way to command chimera via python to crop regions and save them from a density map? I have been using Volume Viewer to define specific regions ('region min max...') and then cropping, and saving. I do this b/c it saves the proper origin for re-insertion into the map at a later time, and models I make with Path Tracer will also be properly oriented in the 'mother tomogram', etc. am I able to do the same thing via py script? have fun on your vacation, -Jeff -- ------------------------------------------------------------- Jeff Triffo Auer Group, Donner Lab, Lawrence Berkeley National Laboratory Raphael Group, Bioengineering Department, Rice University Medical Scientist Training Program (MSTP), Baylor College of Medicine phone (Berkeley): 510-486-7940 fax (Berkeley): 510-486-6488