Thanks Elaine. 

I was omitting the .A. Since they only had one chain I didn't think it was required. But it's working now. 

Best,

Kyle

Dr. Kyle J. Lauersen

Algae Biotechnology & Bioenergy

Center for Biotechnology

Bielefeld University

p: +49 (0) 521 106 12261

e: kyle.lauersen@uni-bielefeld.de


On 29.01.2016, at 20:43, Elaine Meng <meng@cgl.ucsf.edu> wrote:

Hi Kyle,
The following works for me:

I opened YFP and PcPs in that order, then:

commands:
~ribbon
disp @n,ca,c
sel #1:559.a@C #0:2.a@N

menu: Tools….Structure Editing… Build Structure, change to Join Models section of the resulting dialog.

Then I chose the “C-N peptide bond” option and clicked Apply, and the join appears to take place successfully.

However, I see your point that something weird is going on:  I repeated the process several times, and sometimes (but not always!!) if I didn’t change from ribbon to atom display, the Apply button was grayed out.  We should look into why that is happening.

I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.                       
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
Department of Pharmaceutical Chemistry
University of California, San Francisco



On Jan 29, 2016, at 5:33 AM, Kyle J. Lauersen <kyle.lauersen@uni-bielefeld.de> wrote:

Hello,

I am trying to join the two models attached to this sequence.

I have used the sequence selection code in atom specifier: #1:559.a@C #0:2.a@N

Where model #0 is YFP and #1 is PcPs.

When I go to join models, the Apply button is not accessible.

Can you indicate if I am doing something incorrect in the selection of the C and N atoms?

Thank you for your time.

Kyle

<YFP.pdb>
<PcPs.pdb>

Dr. Kyle J. Lauersen
Algae Biotechnology & Bioenergy
Center for Biotechnology
Bielefeld University
p: +49 (0) 521 106 12289
e: kyle.lauersen@uni-bielefeld.de

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