Dear Francesco, Chimera does not have any option to create a lipid bilayer. If you have a bilayer structure already, open as a different model, you can move the protein and bilayer individually by activating/deactivating them for motion. Then you would have to delete lipid molecules that overlap the protein manually before saving to a file. In other words, there is no automatic embedding of a structure in a membrane or a solvent box. about activating/deactivating for motion: http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/mouse.html#activedef about writing a PDB file: http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/savemodel.html#pdb Note even if you save to a single file, the models will still be treated as separate models. You would still have to manually edit the file to get everything into one model, avoiding use of duplicate atom numbers, etc. Best Elaine ----- Elaine C. Meng, Ph.D. meng@cgl.ucsf.edu UCSF Computer Graphics Lab and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco http://www.cgl.ucsf.edu/home/meng/index.html On Nov 18, 2007, at 8:26 AM, Francesco Pietra wrote:
I have installed chimera-1.2470 on Debian Linux i386. Thank you. I want to insert a protein into a lipid membrane in a precise opientation, then save the protein pdb in this configuration. I used VMD in the last few days to this redard. I wonder whether I can stick to Chimera for this purpose too. I tried by selecting "protein", though it was difficult to proceed. Thanks francesco pietra