Hi Bobo, I think Chimera does not have such capability. I would suggest you trying with FoldX: foldx.crg.es Best, Aldo 2014-10-07 17:04 GMT-04:00 <bobo2412@gmail.com>: Hi, Do you know if chimera can do energy calculation? I have a protein I want to mutate some residues and calculate the energy difference before and after the mutation. Do you know if I can do that in chimera? Thanks, Bobo 发自我的 iPhone _______________________________________________ Chimera-users mailing list Chimera-users@cgl.ucsf.edu http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users -- ========================================= Aldo Segura-Cabrera Research Fellow Division of Experimental Hematology and Cancer Biology Cancer and Blood Diseases Institute Cincinnati Children's Hospital Medical Center 3333 Burnet Ave, MLC 7013, Cincinnati OH 45229 e-mail: Aldo.Segura-Cabrera@cchmc.org; aldosegura@gmail.com =========================================