Perfect. Thanks very much. This is what I needed to know. Jonathan Schaefer On Wed, Nov 18, 2009 at 10:36, Tom Goddard <goddard@cgl.ucsf.edu> wrote:
Hi Jonathan,
The Chimera exported STL coordinates will have units of Angstroms since those are the units of the PDB file. So the STL produced by exporting the two chains to separate files will have matching scale. You have to be sure when you send the print job to the printer that you specify the same scale factor. If you are using a uPrint 3-d printer it has a field in the print job setup software to specify a scale.
There have been problems with molecular model ball and stick / ribbon depictions exported as STL not working correctly on the uPrint printer. The model falls apart I think. Greg Couch on the Chimera team knows more about this including the current status. Exported surfaces have printed without any problems.
Here is a description and examples of plastic models we printed using Chimera:
http://www.cgl.ucsf.edu/Outreach/technotes/uprint.html
http://www.cgl.ucsf.edu/Outreach/technotes/ModelGallery/index.html
Tom
-------- Original Message -------- Subject: [Chimera-users] control of scale in STL output files From: Jonathan Schaefer To: chimera-users Date: 11/18/09 9:02 AM
Hi-
I've got a question about controlling the scale of protein models in .stl files generated by Chimera under File/Export Scene in the GUI.
I'm trying to print a 3D model of two interacting proteins with a rapid prototyping machine that takes .stl files as input. The two proteins are co-crystalized and contained in one .pdb file (1nbf). I want the two proteins to be printed at the same scale so that I could fit them together like legos. Can I achieve this by getting a scale set, then showing just the first chain and exporting the data to a .stl file, followed by showing just the other chain and exporting the data to a second .stl file?
I'm a novice to Chimera, so I might be missing something obvious. Any help you offer will be appreciated.
Jonathan Schaefer Morgan Lab UCSF