Hi Charles,
Alternatively, you can also do this with the graphical interface.  Just add each conformation twice when you are adding conformations.  Then, choose the second conformation in the final list you've made (i.e. the second copy of your first conformation) and change "interpolation steps" to 15 (for a half-second pause).  Then choose the third state and change the interpolation steps to however many you want when it's moving.  Then choose the fourth state and use 15 again, etc.
I notice that the final list of conformations wants you to click in with the mouse to change which one you're working on -- the up/down arrows keys seem to change the conformation chosen but don't update the rest of the interface, so beware of that.

--Eric
 
                        Eric Pettersen
                        UCSF Computer Graphics Lab
                        http://www.cgl.ucsf.edu

On May 4, 2011, at 12:57 PM, Elaine Meng wrote:

Sigh, the coordset commands in my previous reply are not quite right, you would put the model number before the frames.  Sorry about that.  However, the commands are all correct within the "trmovie" example command file discussed in that message.
Elaine

On May 4, 2011, at 12:51 PM, Elaine Meng wrote:

Hi Charles,
It can be done with commands.  First you would create the morph trajectory, then use a Chimera command script (just open a plain text file containing the commands) of the general form:

movie record [options]
coordset 1,20 #3; wait 20
wait 10
coordset 21,40 #3; wait 20
wait 10
coordset 41,60 #3; wait 20
movie stop
movie encode [options]

where [options] is not meant literally but would give your desired movie settings, and you would also change other things depending on which model is your trajectory and which frames cover the transitions, and how long you would like the pauses to be.  I would also recommend saving the session with your morph trajectory in the desired orientation so you can revisit it later and easily re-make the movie with any needed variations.

There is an example command file showing a similar procedure...
the "trmovie" example includes morphing and uses coordset to play parts of the trajectory and does other stuff between the uses of coordset (it actually does the morph creation too, but it may be easier for you to do that part beforehand):
<http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/movies.html#examples>

... and the resulting movie is in the Animation Gallery, currently third on this page:
<http://www.cgl.ucsf.edu/chimera/animations/animations.html>

More info on movies and movie-related commands:
<http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/movies.html>
<http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/movies.html#moviecommands>

I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.                       
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
Department of Pharmaceutical Chemistry
University of California, San Francisco

On May 4, 2011, at 11:51 AM, Charles Greenberg wrote:

I have a 3-state structure and I'm currently using morph conformations  
to interpolate between them.  This works very nicely, but I'd like the  
output movie to pause for half a second (a few frames) during each of  
the three key states.  Anyone know how to do this?  I know you can add  
behaviors during particular frames, but I don't know how to make it  
pause.  Thanks!
--Charles


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