On Aug 26, 2022, at 4:51 AM, Wayne Lim <wayne.lim@student.manchester.ac.uk> wrote:

Dear Tom,

I just tried this and over multiple times chimera 1.6 crashed and returned this error message:

chimera exited abnormally, the exit code was: -1073741819

Not sure why it's doing that, any ideas as to what that code means?

BW,

Wayne

---

From: Tom Goddard <goddard@sonic.net>
Sent: Thursday, August 25, 2022 11:50 PM
To: Wayne Lim <wayne.lim@student.manchester.ac.uk>
Cc: chimera-users@cgl.ucsf.edu <chimera-users@cgl.ucsf.edu>
Subject: Re: [Chimera-users] Chimera ac Im command not working

 

Hi Wayne,

  I guess you mean you tried the current Chimera version 1.16.  To change the sign of the map values use command

vop scale #0 factor -1

I don't know what is wrong if the graphics is black after that, but I would start looking at the histogram in the Volume Viewer window and see if the yellow brightness curve is in the right place.  Below is an image with Chimera 1.16 and map EMDB 1273 showing the histograms for the original and inverted map and it displays correctly on my Mac.

Tom

<invertmap.png>

On Aug 25, 2022, at 8:39 AM, Wayne Lim via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:

Dear Chimera team,

 

I am using Chimera to view the surface pane of my TEM images in attempts to see the features of the image in 3D. Since I stained it positively, I have to invert the map values so I can properly view it under “surface”. However, when I do so in Chimera 1.5, it displays an “unexpected python error”. When tried in Chimera 1.6, the result is simply a black screen. I am stumped as to how to solve this issue. A colleague of mine done it and I followed the same procedure he has done, but to no avail.

 

Best,

Wayne 

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