1. open *mrc
put the map to Level 0.011
2. vop gaussian #0 sd 0.005
put Gaussian to 0.011
3. Go to: Tools --> Volume Data --> Segment Map
Segment the gaussian map
Smoothing step: 1
Step size: 500 voxels
Keep only regions at lease: 100 voxels
Save segments as an .mrc file
Go to: File (Segment Map) --> Save all regions to .mrc
Set Segment map to 0.011
4. Type: vop subtract #3 #0
#3 Corresponds with ID# of the segmented .mrc file
#0 Corresponds with ID# of the original file
5. sop split #0
#0 Corresponds with ID# of the original file