Dear Elaine,

Thank you for your email. So I build missing residues for my both chains separately, is there a way to combine these two chains after that process?? I mean, I have now two chains which they are both the outputs of Modeller, how to create a single PDB out of two processed PDB.

Cheers,

On Tue, Apr 4, 2017 at 6:42 PM, Elaine Meng <meng@cgl.ucsf.edu> wrote:

Dear Armin,
Sorry, the Chimera-Modeller interface does not allow modeling more than one chain. Modeling a multimer or complex of multiple chains requires running Modeller separately.

<http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/multalignviewer/modeller.html>

Regards,
Elaine
----------
Elaine C. Meng, Ph.D.
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
Department of Pharmaceutical Chemistry
University of California, San Francisco

> On Apr 4, 2017, at 4:56 AM, ARMIN HODAEI <ahodaei15@ku.edu.tr> wrote:
>
> Dear Chimera Users,
> I want to use refining loop option to build missing residues for my target protein. First I get the FASTA file structure of protein then I load it into Chimera, after that in the Structure tab I select load structure, then I see the protein. After that Again in the "Structure" tab I select Modeller(loops/refinement). The rest is easy for creating the new structure PDB. But the problem is that I want whole protein to be treated in this refinement, but it only chose one chain, How can I do this for both chains at once?
> Sincrely,
> --
> Armin Hodaei
> Department of Physics,
> Faculty of Arts and Sciences
> Koc University, Istanbul

Armin Hodaei
Department of Physics,
Faculty of Arts and Sciences
Koc University, Istanbul