I spent some time doing this recently. Chimera is a wonderful tool for this purpose. There is a very helpful (and short!) tutorial you should work through: http://www.cgl.ucsf.edu/Outreach/Workshops/UCSF-Fall-2005/07-VolumeData/tuto... There is a sort of local search tool for fitting models to density, although I think you need ot have pretty good data for it to work. On 6/11/06, Puey Ounjai <pounjai@gmail.com> wrote:
Dear all,
I am trying to dock atomic coordinate of a protein derived from 3D X-ray crystal (PDB format) into lower resolution (about 20-25 A resolution) EM map (BRIX, XPLOR ASCII or SITUS format) from 2D crystal.
1. Does Chimera have this kind of fitting tool (PDB to volume) or I have to fit the coordinate by hand? If chimera can do this task, Which command should I use? 2. Does Chimera have any statistical evaluation for manual fitting? 3. How can you evaluate the % matching of 2 models in Chimera?
Best regards, Puey Ounjai _______________________________________________ Chimera-users mailing list Chimera-users@cgl.ucsf.edu http://www.cgl.ucsf.edu/mailman/listinfo/chimera-users