Dear Chimera developers,

I’m working on a protein complex and I’d like to analyse the SASA and hydrophobicity on the surface.

I have worked with Chimera before so I know how to do those basic tasks for a monomeric protein; however, since I have five monomers interacting together I was wondering how to calculate the SASA (and later showing the hydrophobic residues of the surface) excluding the monomer-monomer interfacial regions.

I would also need those values tabulated to show hydrophobicity vs primary sequence but still excluding the monomer-monomer interfaces!

It seems like there is no basic tool to do that (or am I missing something?); is there any procedure I could apply?

Unfortunately I can’t code in Python so I’m looking for a solution that involves using the GUI.

Thank you,

Best regards,

Alessandro Strofaldi, PhD

University of Bristol