Hi Tom, I am currently exploring sample heterogeneity using tomographic approach on single particles. Because of the tomography I have a missing cone in the angular information, so my structures are distorted in the Z direction. To obtain a reasonable structure I have to average the similar structures and I want to do it in IMAGIC. My thought is to average the projections of several structures in the same direction and rebuild the model. Therefore I need to align the different starting density maps in rotation and translation also. Since this feature is not working very well in IMAGIC I would like to use Chimera, but I need the transformation values (angles and shifts) after the fitting, to apply it on the density maps. It would also help me a lot if you tell me how I can obtain these informations! Thanks in advance! Bye, Gabor -- Gabor Papai IGBMC Department of Structural Biology and Genomics 1, rue Laurent Fries, BP 10142 67404 Illkirch, France phone +33-3-90244796 Fax +33-3-88653201 E-mail: papai@igbmc.u-strasbg.fr