Hi Tom, Thanks for your response and the info. Thinking about it, maybe one could create a series of volumes and mask away the density of the symmetric copies so that you’re left with the asymmetric unit? Kyle
On 3 Jun 2016, at 13:26, Tom Goddard <goddard@sonic.net> wrote:
Hi Kyle,
If you have a point symmetry, e.g. icosahedral symmetry, Chimera has no way to extract a wedge of density that is one asymmetric unit. If you have an atomic model of an asymmetric unit fit into the density, you could extract a box around it using Chimera volume viewer menu entry Features / Atom Box. Or you could extract the density just at grid points within a specified distance of atoms using volume viewer menu entry Features / Zone…. To save the results of either of those methods use volume viewer menu File / Save Map As….
Tom
On Jun 3, 2016, at 11:41 AM, Morris, Kyle wrote:
Hi Chimera team and users,
Is there a way to extract the asymmetric unit from a volume which has been 'symmetrised’ from, say for instance, Relion? In this case what are the conventions used by chimera to define the bounds of the sub-volume to extract?
Thanks for your help!
Best wishes, Kyle
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