Dear List,

I am trying to use UCSF chimera to make a movie after TEM reconstruction using IMOD. But an error message always pops out, even I use the script in the examples. Belows shows the error messages in the Reply Log. Can anyone take a look and give me some clue where the problem is?

Thank you.

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C:\Program Files\Chimera 1.8.1\bin\ffmpeg.exe -r 25 -i c:\users\337\appdata\local\temp\chimovie_zUrR-%05d.ppm -vf crop=(in_w/2)*2:(in_h/2)*2:0:0 -y -vcodec libx264 -f mp4 -vb 200.0k C:\Users\Public\Desktop\rock.mp4

An error occurred during encoding. See Reply Log for details.

Error during MPEG encoding:

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Exit value:    1

Error message: 

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C:\Program Files\Chimera 1.8.1\bin\ffmpeg.exe -r 25 -i c:\users\337\appdata\local\temp\chimovie_zUrR-%05d.ppm -y -vcodec libvpx -f webm -vb 200.0k C:\Users\Public\Desktop\rock.webm

An error occurred during encoding. See Reply Log for details.

Error during MPEG encoding:

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Exit value:    1

Error message: 

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C:\Program Files\Chimera 1.8.1\bin\ffmpeg.exe -r 25 -i c:\users\337\appdata\local\temp\chimovie_zUrR-%05d.ppm -y -vcodec libtheora -f ogg -vb 200.0k C:\Users\Public\Desktop\rock.ogv

An error occurred during encoding. See Reply Log for details.

Error during MPEG encoding:

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Exit value:    1

Error message: 

Thank you very much,

Wenda

Wenda Wang,

PhD candidate

Department of Materials Sciences and Engineering,

Drexel University, Philadelphia, PA

215-6883348