Dear Elaine, Thank you for your reply. When I am trying to fit model using reference pdb. I am not getting appropriate response. Because my reference pdb has only 11 chains while my protein has more than this. Kindly suggest some person who can help me with this. I will be heartily thankful to you Regards Neha Goel On Mon, 10 May 2021, 20:51 Elaine Meng, <meng@cgl.ucsf.edu> wrote:
Hi Neha, An MRC file is a density map format. You cannot save it as an atomic model, because it does not contain atomic coordinates.
There is no tool in Chimera to create atomic structure instantly from a map. Researchers use various programs to build atomic structures into a map or to build an atomic model separately and then fit it into a map, but it is generally an interactive process that takes many steps. I am not an expert on this process, but you could read hundreds of research papers "Methods" sections to see descriptions of the ways in which it is done.
Regards, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On May 10, 2021, at 10:24 AM, Neha Goel <nehagoel24march@gmail.com> wrote:
Dear Chimera, I have a .mrc file. I reopened the file in chimera and would like to save it in pdb. I am unable to save it. It needed the molecular model pdb. Kindly guide me the way to save it. Regards Neha Goel