I am not an expert, but I think you can load a PDB file and select the residue you want to mutate. Then Tools > Structure Editing > Rotamers. A nice tutorial: https://www.youtube.com/watch?v=bcXMexN6hjY Regards. On Thu, Apr 2, 2020 at 5:37 PM Teresa Brito-Robinson < Teresa.Brito-Robinson.2@nd.edu> wrote:
Hi Chimera users,
I am trying chimera for the first time and my question is:
- How can I change (substitute) specific amino acids within an uploaded protein sequence to mimic a missense mutation?
- Or how can I enter a whole new custom made protein sequence into chimera, so I can probe different variants of a protein sequence.
I don't know how to make the file like pdb (protein database) so I can fetch my custom sequence?
Thanks!
Teresa _______________________________________________ Chimera-users mailing list: Chimera-users@cgl.ucsf.edu Manage subscription: http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users