Dear all,

 

I have a few volumetric cryo EM Maps of the same protein in different conformations. And I would like to morph between those conformations. However, the volumes are not perfectly aligned in first place. So I align all of them on one of the maps using the “fit in map” functionality.  This works great and when I look through the different structures with the volume viewer, they are perfectly aligned. However, if I morph through the maps using the following command:

 

vop morph #0-19 playstep 0.003 constantVolume true frames 1000   

 

the maps are morphed in their original position so the morph is quite jumpy.

 

Is there a way to morph through them in the aligned coordinate system directly?    

 

Kind regards

 

David

 

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Dr. David Haselbach

Max-Planck-Institut für biophysikalische Chemie

AG Stark

Am Fassberg 11

D-37077 Goettingen

dhaselb@gwdg.de

+49-551-2011302