Hi Elaine, Thank you for your response! I tried using the command and I have been trying two maps which looks very similar, but with different resolution (3.6 A (yellow) vs 3.2 A (grey)) and with different pixel size (1.05 vs 0.822). Will this affect the gridding? (I am not very familiar with what you mean by the same grid). Best, Yangqi Sent from Mail for Windows 10 From: Elaine Meng Sent: Friday, November 16, 2018 7:45 PM To: Yangqi Gu Cc: chimera-users Subject: Re: [Chimera-users] Difference map Hi Yangqi, Yes, see command “vop subtract”: <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html#subtract> Keep in mind that if the two aren’t on the same grid, there may be small differences due to the need to sample them at the same points to do the subtractions. I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Nov 16, 2018, at 4:39 PM, Yangqi Gu <yangqi.gu@yale.edu> wrote:
Dear Chimera users, I am trying to examine two cryo-EM maps and want to know if there is a subtle change between these two. I want to calculate the difference map, is it doable in Chimera? Best, Yangqi