Hi Chimera users,

I am trying chimera for the first time and my question is:

How can I change (substitute) specific amino acids within an uploaded protein sequence to mimic a missense mutation?

Or how can I enter a whole new custom made protein sequence into chimera, so I can probe different variants of a protein sequence.

I don't know how to make the file like pdb (protein database) so I can fetch my custom sequence?

Thanks!

Teresa