Hi Milad,

An easy way to make a fusion protein is to use ChimeraX and run AlphaFold to predict the structure.  AlphaFold is not available in our old Chimera program -- no new features have gone into Chimera in over 5 years and many new capabilities are in ChimeraX.

In ChimeraX, use menu Tools / Structure Prediction / AlphaFold, paste in your fusion protein sequence and press Predict.  You may need to log into your Google account since the calculation is run on Google Colab servers.  Calculation time is 5 minutes to a few hours depending on sequence length.

Tom


On Aug 4, 2022, at 7:40 AM, Eric Pettersen via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:



Begin forwarded message:

From: Milad Mapar <milad.mapar@yahoo.com>
Subject: Making Fusion Proteins using Chimera
Date: August 4, 2022 at 6:26:46 AM PDT

Dear Eric

Hello

With thanks to your support on Chimera, could you please help me to make a Fusion Protein using Chimera 1.16

Any links to tutorials or hints would be appreciated 

Many thanks 

Pharmaceutical Biotechnology PhD Student
Milad 

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